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The newly developed Toluidine Blue and Eosin Y (T & E) staining
is the optimum staining method in functional genomics.
The method allows immediate fixation with ethanol and non-
aqueous staining/washing method without any RNA degradation.
It is a simple and unique method with good optical resolution.
 
       Ethanol fixation
       Isopropanol solution
       Toluidine blue and Isopropanol (with urea) solution
       Ethanol washing
       Eosin Y and Isopropanol solution
       Isopropanol washing